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KMID : 0370219830270020117
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Yakhak Hoeji
1983 Volume.27 No. 2 p.117 ~ p.124
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Development of Homogeneous Enzyme Immunoassay for Serum 3,5,3’’-Triiodothyronine Determination
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이희주/Lee HJ
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Abstract
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For development of EMIT-T3 assay, the conjugation of 3, 5, 3’’-triiodothyroformic acid NHS ester to G6PDH was attempted in various reaction conditions. Up to now, the best conjugation condition was the ratio of T3-NHS:G6PDH=100 in 25% carbitol-Tris buffer at pH 9, OoC during overnight. The obtained T3-G6PDH conjugates usually had 20% residual enzyme activity which was inhibited by 40~70% with various anti-T3 antibodies. Utilizing the conjugate I and an antibody (S2633G), a useful standard curve for T3 assay was obtained in the range of 0 to 5ng/ml with 499 EMIT units of separation.
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KEYWORD
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